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Separation sciences, in all their various forms such as chromatography, field-flow fractionation, and electrophoresis, provide some of the most powerful techniques in analytical chemistry and are applied within a number of important application areas, including archaeology, biotechnology, clinical, environmental, food, medical, petroleum, pharmaceutical, polymer and biopolymer research. Beyond serving analytical purposes, separation techniques are also used for preparative and process-scale applications. The scope and power of separation sciences is significantly extended by combination with spectroscopic detection methods (e.g., laser-based approaches, nuclear-magnetic resonance, Raman, chemiluminescence) and particularly, mass spectrometry, to create hyphenated techniques. In addition to exciting new developments in chromatography, such as ultra high-pressure systems, multidimensional separations, and high-temperature approaches, there have also been great advances in hybrid methods combining chromatography and electro-based separations, especially on the micro- and nanoscale. Integrated biological procedures (e.g., enzymatic, immunological, receptor-based assays) can also be part of the overall analytical process.

分離科學，包括色譜法、場流分離和電泳等各種形式，提供了分析化學中最強大的一些技術，并應用于許多重要的應用領域，包括考古學、生物技術、臨床、環境、食品、醫療、石油、制藥、聚合物和生物聚合物研究。除了用于分析目的外，分離技術還用于制備和工藝規模應用。分離科學的范圍和能力通過與光譜檢測方法（例如，基于激光的方法、核磁共振、拉曼、化學發光）和特別是質譜法相結合而大大擴展，從而創建了聯用技術。除了色譜法中令人興奮的新發展，例如超高壓系統、多維分離和高溫方法，在結合色譜法和電分離的混合方法方面也取得了巨大進展，尤其是在微米和納米尺度上。綜合生物學程序（例如酶促、免疫學、基于受體的分析）也可以成為整體分析過程的一部分。

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